Interstate 85 begins as a fork off Interstate 65 in Montgomery. From here, I-85 parallels U.S. Route 80 until the highway nears Tuskegee. At Tuskegee, I-85 leaves U.S. 80 and starts to parallel U.S. Route 29, which the highway parallels for much of its length.
I-85 also passes near Auburn, Opelika and Lanett before crossing the Chattahoochee River into Georgia.